| Reverse cholesterol transport: Novel insights Prof. dr. Bert Groen Universitair Medisch Centrum Groningen |
| | Definition of reverse cholesterol transport (RCT) |
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| Simplified scheme of cholesterol homeostasis |
| | Classic scheme of reverse cholesterol transport |
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| Disruption of Acba1 should have a drastic effect on fecal neutral sterol excretion |
| | Disruption of Abca1 in mice has no effect on neutral sterol excretion in feces |
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| Determination of tissue cholesterol efflux using 13C-Cholesterol infusion (Kinemed method) |
| | Classic scheme of reverse cholesterol transport |
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| About 40% of tissue cholesterol efflux in humans is HDL dependent |
| | Summary I |
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| Intestine perfusion |
| | Intestinal perfusion to measure direct cholesterol secretion |
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| Trans Intestinal Cholesterol Excretion (TICE) |
| | Transintestinal cholesterol secretion (TICE)= transport directly from blood to intestinal lumen |
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| In vivo determination of TICE in mice is straightforward. |
| | Summary II |
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| In vivo determination of TICE in humans is complicated because biliary cholesterol output has to be quantified |
| | Estimation of biliary cholesterol output |
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| Study protocol |
| | Simplified model of cholesterol fluxes |
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| Summary III |
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